Journal: Neurophotonics

Article Title: Review of optical coherence tomography based angiography in neuroscience

doi: 10.1117/1.NPh.3.1.010902

Figure Lengend Snippet: (a) and (b) OCT angiography images of mouse cerebral cortex through skull using UHS-OMAG. Images are shown in en face maximum intensity projection (MIP) of volumetric angiography data.52 (c) The depth-projected en face MIP of volumetric ...

Article Snippet: Recently, Gagnon et al. 62 combined two-photon laser scanning microscopy (TPLSM) angiography with Doppler OCT to acquire a more accurate 3-D volumetric blood flow map by reconstructing the TPLSM angiogram from the model with constraints from the Doppler OCT measurements [ – ].

Techniques:

Journal: Neurophotonics

Article Title: Review of optical coherence tomography based angiography in neuroscience

doi: 10.1117/1.NPh.3.1.010902

Figure Lengend Snippet: (a) RBC flux in rat cortex capillaries is shown as color spots on the en face MIP of the 3-D angiogram.45 Flux is estimated by monitoring the time course of the OCT intensity signal. (b) 3-D RBC flux map of the capillary network in rat ...

Article Snippet: Recently, Gagnon et al. 62 combined two-photon laser scanning microscopy (TPLSM) angiography with Doppler OCT to acquire a more accurate 3-D volumetric blood flow map by reconstructing the TPLSM angiogram from the model with constraints from the Doppler OCT measurements [ – ].

Techniques:

Journal: Neurophotonics

Article Title: Review of optical coherence tomography based angiography in neuroscience

doi: 10.1117/1.NPh.3.1.010902

Figure Lengend Snippet: Volumetric OCT angiography imaging of the cortex during ischemic stroke. En face MIP of UHS-OMAG images (a) during baseline, (b) progressive focal ischemia developed during MCAO, and (c) 30 min after onset of reperfusion. ...

Article Snippet: Recently, Gagnon et al. 62 combined two-photon laser scanning microscopy (TPLSM) angiography with Doppler OCT to acquire a more accurate 3-D volumetric blood flow map by reconstructing the TPLSM angiogram from the model with constraints from the Doppler OCT measurements [ – ].

Techniques: Imaging

Journal: Neurophotonics

Article Title: Review of optical coherence tomography based angiography in neuroscience

doi: 10.1117/1.NPh.3.1.010902

Figure Lengend Snippet: En face MIP of volumetric OCT angiography images of the cortex during TBI in mice in vivo at different time points.73 Compared to baseline (a), progressive vessel regulation (vessels indicated by white arrows) and neovascularization at traumatic areas ...

Article Snippet: Recently, Gagnon et al. 62 combined two-photon laser scanning microscopy (TPLSM) angiography with Doppler OCT to acquire a more accurate 3-D volumetric blood flow map by reconstructing the TPLSM angiogram from the model with constraints from the Doppler OCT measurements [ – ].

Techniques: In Vivo

Journal: Neurophotonics

Article Title: Review of optical coherence tomography based angiography in neuroscience

doi: 10.1117/1.NPh.3.1.010902

Figure Lengend Snippet: Representative OCT angiography images of the cortex within the MCA territory at baseline, 1 h post-SAH/sham (pretreatment) and 24 h post-SAH/sham (posttreatment).78 tPA reduces ICP and improves cortical perfusion 24 h after SAH. ...

Article Snippet: Recently, Gagnon et al. 62 combined two-photon laser scanning microscopy (TPLSM) angiography with Doppler OCT to acquire a more accurate 3-D volumetric blood flow map by reconstructing the TPLSM angiogram from the model with constraints from the Doppler OCT measurements [ – ].

Techniques:

Journal: Frontiers in Immunology

Article Title: Corrigendum: Central role of PD-L1 in cardioprotection resulting from P2Y 4 nucleotide receptor loss

doi: 10.3389/fimmu.2022.1061958

Figure Lengend Snippet: Identification of PD-L1 as a marker of beige adipocytes, upregulated in P2Y 4 KO PAT. (A) Quantification of lipid droplet size in adipocytes generated from WT, P2Y 4 KO, Adipo KO and DKO cADSCs in the presence or absence of R+I treatment. Quantification was performed using ImageJ software (n = 4). (B) Oil red O staining of cardiac adipose-derived stem cells (cADSC) cultures after adipogenic differentiation and after treatment with two adipocyte browning agents, rosiglitazone (1 µM) plus isoproterenol (100 µM) (R+I) (200x magnification). Adipogenic differentiation leads to big lipid droplet appearance in control (Ctrl) cADSC cultures. The additional presence of browning agents (R+I) in the differentiation medium leads to small lipid droplet appearance in cADSC cultures. (C) Higher PD-L1 expression in P2Y 4 KO differentiated cADSCs. Density plots, percentage of PD-L1 positive cells and mean fluorescence intensities (MFI) obtained by flow cytometry quantification of PD-L1 expression in differentiated adipocytes isolated from PAT of WT, P2Y 4 KO, Adipo KO and DKO mice, with or without R+I (n = 4-6). (D) Flow cytometry analysis showing increased level of PD-L1 expression in P2Y 4 KO PAT. Density plots, percentage of PD-L1 positive cells and mean fluorescence intensities (MFI) detected by flow cytometry in PAT of sham (no MI) or ischemic (MI) WT, P2Y 4 KO, Adipo KO and DKO mice, 24h post-MI (n = 5-7). (E, F) . Loss of P2Y 4 leads to increased PD-L1 immunohistological staining in ischemic PAT. (E) Immunofluorescent stainings of perilipin lipid droplet marker, and PD-L1 performed on optically cleared whole-mounted PAT of WT, P2Y 4 KO, Adipo KO and DKO ischemic mice, 24h post-MI (5X magnification). (F) Representative confocal microscopy reconstructions of stained and optically cleared whole-mounted PAT (20X magnification).

Article Snippet: Two-photon laser scanning microscopy (TPLSM) reconstructions of perilipin and PD-L1 stainings were also made at higher magnification on PAT sections of ischemic mice, 24h post-MI ( ).” The corrected sentences appear below: “Immunohistological stainings of perilipin, a lipid droplet marker, and PD-L1 were then performed on whole-mounted PAT of WT, P2Y 4 KO, adiponectin KO and DKO ischemic mice, 24h post-MI ( ).

Techniques: Marker, Generated, Software, Staining, Derivative Assay, Control, Expressing, Fluorescence, Flow Cytometry, Isolation, Confocal Microscopy